American Society of Hematology

Fig. 5.

$Fig. 5. eGFP-specific CTL activity is mediated by CD8+ lymphocytes and is MHC restricted. / (A) Stimulated PBMCs from Mm 96-97 were obtained at 25 weeks after transplantation, separated into CD4+ and CD8+fractions using immunomagnetic beads, and then examined for eGFP-specific CTL activity using an eGFP-expressing autologous B-LCL. (B) Stimulated PBMCs from Mm 256-97 were examined for their ability to lyse eGFP-transduced (+) or -untransduced (−) autologous and allogeneic B-LCLs.$

eGFP-specific CTL activity is mediated by CD8⁺ lymphocytes and is MHC restricted.

(A) Stimulated PBMCs from Mm 96-97 were obtained at 25 weeks after transplantation, separated into CD4⁺ and CD8⁺fractions using immunomagnetic beads, and then examined for eGFP-specific CTL activity using an eGFP-expressing autologous B-LCL. (B) Stimulated PBMCs from Mm 256-97 were examined for their ability to lyse eGFP-transduced (+) or -untransduced (−) autologous and allogeneic B-LCLs.

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