CD4⁺Vα24NKT cells derived from a patient with AML M4 were phenotypically and functionally similar to those from HDs.

(A) A representative immunophenotype of Vα24NKT cell line obtained from Pt 1 with AML M4. The Vα24NKT cells consistently express CD4, Vα24, Vβ11, and CD161. (B) Cytotoxic activity of activated CD4⁺Vα24NKT cells from Pt 1 against U937 (■), K562 (▵), and Daudi (●) target cells tested by the 4-hour⁵¹Cr release assay. CD4⁺Vα24NKT cells were used as effector cells on day 3 after stimulation by α-GalCer–pulsed DCs. The data indicate cytotoxicity at the E/T ratios of 10:1 and 20:1 using the 4-hour ⁵¹Cr release assay. The results are expressed as mean ± SD (n = 4) of the percentage cytotoxicity.