Apoptotic cell death of PBMCs from AML patients and cord blood CD34⁺ cells following coculture with activated CD4⁺Vα24NKT cells derived from Pt 1.

Target cells were gated for the PBMCs from Pt 1 based on the forward light scatter (FW-SC) and the side light scatter (RT-SC) (A). A similar gating procedure was used for assays using the other targets. Early apoptotic cells are defined by the AV⁺PI⁻population in the dot plots for FITC-AV binding (FL 1 channel) and PI uptake (FL2 channel) (B). The data shown represent 1 of 3 experiments with similar results.