TIMP-1 induces further B-cell differentiation in IL-10–independent manner:

Y axis: Cell number. X axis: Log fluorescence staining with FITC-labeled antibody. Dotted lines indicate staining with isotype control. Solid lines indicate staining with anti-CD77. (A) Expression of CD77 in JD38 cells (do not express TIMP-1 or IL-10 protein). Cells express CD77. (B) Expression of CD77 in TIMP-1 JD38 clone 24 (expresses and secretes TIMP-1 and IL-10). Cells are negative for CD77 (0% positive for CD77). (C) Expression of CD77 in TIMP-1 JD38 clone 24 after 48-hour incubation with neutralizing anti–TIMP-1 antibody (no active TIMP-1). CD77 expression up-regulated. (D) Expression of CD77 in JD38 cells incubated with rIL-10 (no TIMP-1, active IL-10). Cells express CD77 (23% of the cells stain higher with anti-CD77 than with isotype control).