Demonstration of clonotypic PCR before and after treatment in an nPR patient.
(A) Clonotypic PCR product in the initial pretreatment samples of this patient at a 10−4 dilution in the BM after primary PCR. (B) After seminested PCR, the clonotype is detected at a 10−4 dilution with greater intensity confirming the previous primary PCR result. (C) Persistent clonotype PCR after treatment from a nodular PR BM specimen for this same patient. Primary PCR detects a clone at a 10−2 dilution. (D) Seminested PCR confirms the primary PCR finding, detecting a clone at a 10−2 dilution. DNA was extracted from an aspirate corresponding to the bone marrow biopsy shown in Figure 4B. (A-D) Lanes 1-8, serial 10-fold dilutions of patient DNA starting with a 100 ng template; lane 9, negative control with no template DNA; lane 10, 1 ug polyclonal DNA; lane 11, positive control with bcl-2 primers and 100 ng of template.
