Fig. 2.
Fig. 2. Erythropoietin levels are increased in the fetal livers of E14.5 and E16.5 PDGF-B−/− embryos. / Livers from E14.5 and E16.5−/− and +/+embryos were snap frozen and kept at −80°C until analysis after lysis and homogenization in 150 mM NaCl, 66 mM EDTA, 10 mM Tris HCl (pH 7.4), 0.4% Na deoxycholate, 1% NP-40, and protease inhibitor cocktail (P-2714; Sigma). Erythropoietin activity was quantified using a combination of 2 commercially available erythropoietin enzyme-linked immunosorbent assay kits.26 Erythropoietin concentration was normalized to the total amount of protein in the sample using the Bio-Rad protein assay. The 2-tailed paired Student t test was used for statistical calculations (P = .023* andP = .030‡). Three animals of each genotype and age were tested. Error bars in the figure represent ±1 SD.

Erythropoietin levels are increased in the fetal livers of E14.5 and E16.5 PDGF-B−/− embryos.

Livers from E14.5 and E16.5−/− and +/+embryos were snap frozen and kept at −80°C until analysis after lysis and homogenization in 150 mM NaCl, 66 mM EDTA, 10 mM Tris HCl (pH 7.4), 0.4% Na deoxycholate, 1% NP-40, and protease inhibitor cocktail (P-2714; Sigma). Erythropoietin activity was quantified using a combination of 2 commercially available erythropoietin enzyme-linked immunosorbent assay kits.26 Erythropoietin concentration was normalized to the total amount of protein in the sample using the Bio-Rad protein assay. The 2-tailed paired Student t test was used for statistical calculations (P = .023* andP = .030‡). Three animals of each genotype and age were tested. Error bars in the figure represent ±1 SD.

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