Fig. 1.
LC induces cell death and loss of clonogenicity in U937 cells.
(A) U937 cells (2 × 105 cells/ml) were exposed to LC at increasing concentrations for 24 hours and then either assayed for apoptosis as determined by morphologic analysis of Wright-Giemsa stained cytospins (●) or their self renewal capacity by their ability to form colonies in soft agar (○). (B) U937 cells (2 × 105 cells/ml) were exposed to 1μM LC (○) and LC plus 10 nM BRY (●) for the indicated intervals, and apoptosis was determined by morphologic analysis of Wright-Giemsa–stained cytospins. Exposure to BRY alone induces approximately 8% to 10% apoptosis (not shown). Values represent means for triplicate experiments (± SEM).
