![Fig. 4. The SAPK cascade is not involved in LC/BRY-induced apoptosis. / (A,B) U937 cell lines expressing a dominant negative construct of c-Jun (TAM67: clone 1-1 [left-hatched bar], clone 2-2 [cross-hatched bar]), and their empty vector controls (open bar) were exposed to either vehicle, bryostatin (B; 10 nM), lactacystin (LC; 1 μM) or the combination lactacystin plus bryostatin (LC/B) for 24 hours. Values represent the means for triplicate experiments and are (A) expressed as the percentage of apoptotic cells determined by morphologic analysis or (B) loss of mitochondrial membrane potential, expressed as the percentage of cells exhibiting low levels of DiOC6 uptake. (C) U937 cells were pretreated with the specific p38/RK inhibitor SB203580 (SB; 10 or 20 μM) 30 minutes prior to the addition of LC plus BRY (LC/B). Values represent the means for triplicate experiments (± SEM).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/7/10.1182_blood.v97.7.2105/6/h80710867004.jpeg?Expires=1735560896&Signature=XayAH91ZAUSkuJ7wOt3fLWHJIZGeOVdNmC~vlvRTybGiEZP0msj4zVjVOwCOZ6ra-pBpfRUoNjGc569CQzsIqtIjAxjRYllPYjsEvleENuKATCO-OqC24oAtlEKDIzOrv9VoKoTGG72~eIOYLHEDj-YKeLR19HcfmP-Y5T-4fCPuA~5GnofDybq7IZy-B~wvTe6wpRWsrC0rEYOeJxLefJykyWbkB0bLYT4HBHGVn6GVixiDTkK2BVopciRjWFrczHCGSWjL8n6AbBmquioiMKBlk7HMLFXDOLwMiTYS-0kVN1xcK~2v5HakLSls9ecbehPDu6zyq4Vo68dHkWFbPw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The SAPK cascade is not involved in LC/BRY-induced apoptosis.
(A,B) U937 cell lines expressing a dominant negative construct of c-Jun (TAM67: clone 1-1 [left-hatched bar], clone 2-2 [cross-hatched bar]), and their empty vector controls (open bar) were exposed to either vehicle, bryostatin (B; 10 nM), lactacystin (LC; 1 μM) or the combination lactacystin plus bryostatin (LC/B) for 24 hours. Values represent the means for triplicate experiments and are (A) expressed as the percentage of apoptotic cells determined by morphologic analysis or (B) loss of mitochondrial membrane potential, expressed as the percentage of cells exhibiting low levels of DiOC6 uptake. (C) U937 cells were pretreated with the specific p38/RK inhibitor SB203580 (SB; 10 or 20 μM) 30 minutes prior to the addition of LC plus BRY (LC/B). Values represent the means for triplicate experiments (± SEM).
