Depletion of Raf-1 opposes LC/BRY-induced apoptosis.

U937 cells were exposed to 1 μM geldanamycin for 18 hours before the addition of either vehicle (C), BRY (B; 10 nM), LC (1 μM), or LC plus BRY (LC/B). Inset indicates the level of Raf-1 expression in cells after 18 hours and before drug treatments. The bar graph illustrates the extent of apoptosis at 24 hours after treatment as determined by morphologic analysis. Values represent the means for triplicate experiments (± SEM).