American Society of Hematology

Fig. 4.

$Fig. 4. Membrane-proximal 10 amino acids from the Mpl intracellular domain are required for TPO-induced proliferation in primary megakaryocyte-lineage cells. / (A) Growth curves of unfractionatedGPIIb-tva+mpl−/− bone marrow after infection with RCAS-Mpl, RCAS-MplΔ7, and RCAS-MplΔ7, Y582F, and selection in TPO demonstrate a loss of TPO-induced proliferation when the membrane-proximal 10 amino acids are missing from the Mpl intracellular domain. (B) All virally expressed Mpl have an N-terminal FLAG epitope. FACS analysis using isotype control (top panels) and anti-FLAG antibody (lower panels) demonstrates similar levels of surface Mpl expression for Baf/3-TVA cells infected with the same virus stocks used in panel A.$

Membrane-proximal 10 amino acids from the Mpl intracellular domain are required for TPO-induced proliferation in primary megakaryocyte-lineage cells.

(A) Growth curves of unfractionatedGPIIb-tva⁺mpl^−/−bone marrow after infection with RCAS-Mpl, RCAS-MplΔ7, and RCAS-MplΔ7, Y582F, and selection in TPO demonstrate a loss of TPO-induced proliferation when the membrane-proximal 10 amino acids are missing from the Mpl intracellular domain. (B) All virally expressed Mpl have an N-terminal FLAG epitope. FACS analysis using isotype control (top panels) and anti-FLAG antibody (lower panels) demonstrates similar levels of surface Mpl expression for Baf/3-TVA cells infected with the same virus stocks used in panel A.

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