Fig. 5.
s-FKN stimulates PI3K activity.
MonoMac6 cells, untreated or, as indicated in the figure, preincubated 2 hours with 10 μM LY294002 or 16 hours with 100 ng/mL pertussis toxin (PTX) or 4 hours with 10 μg/mL cholera toxin (CTX), were exposed to 60 nM s-FKN following different time courses. Cell lysates were immunoprecipitated with antiphosphotyrosine monoclonal antibody and immune complexes were analyzed for PI kinase activity in the absence (A,C) or presence of 0.2% Nonidet-P40 or 200 μM adenosine (B). The results are representative of 2 experiments.
