American Society of Hematology

Fig. 2.

$Fig. 2. Human hematopoietic cells and their putative micro-environments express components of the Notch signaling pathway. / RT-PCR reactions were performed on cells from hematopoietic micro-environment or purified cell fractions (levels of purity greater than 99%) from a minimum of 3 independent samples. (A) Expression of human Notch receptors 1 and 2, hDelta-1, hDelta-4, hDelta-3, and hKuzbanian by putative hematopoietic micro-environment cells of adult BM stromal cells and HUVECs (n = 3). (B) Expression of hNotch-1, hNotch-2, hDelta-1, hDelta-4, hDelta-3, and hKuzbanian by primitive Lin− cell subsets (CD34+CD38−Lin− and CD34+CD38+Lin−) and mature (CD33+ myeloid cells, CD3+ T cells, and CD19+ B cells) from full-term human cord blood samples (n = 4). RT-PCR reactions were performed on fetal cDNA as a positive control while single transcript copy β-glucuronidase was used to assess the quality and integrity of cDNA templates and provide a lower limit of detection.$

Human hematopoietic cells and their putative micro-environments express components of the Notch signaling pathway.

RT-PCR reactions were performed on cells from hematopoietic micro-environment or purified cell fractions (levels of purity greater than 99%) from a minimum of 3 independent samples. (A) Expression of human Notch receptors 1 and 2, hDelta-1, hDelta-4, hDelta-3, and hKuzbanian by putative hematopoietic micro-environment cells of adult BM stromal cells and HUVECs (n = 3). (B) Expression of hNotch-1, hNotch-2, hDelta-1, hDelta-4, hDelta-3, and hKuzbanian by primitive Lin⁻ cell subsets (CD34⁺CD38⁻Lin⁻ and CD34⁺CD38⁺Lin⁻) and mature (CD33⁺ myeloid cells, CD3⁺ T cells, and CD19⁺ B cells) from full-term human cord blood samples (n = 4). RT-PCR reactions were performed on fetal cDNA as a positive control while single transcript copy β-glucuronidase was used to assess the quality and integrity of cDNA templates and provide a lower limit of detection.

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