UVB-induced apoptosis of IDCs is inhibited by zVAD.
(A) IDCs were left untreated, exposed to 1 J/cm2 UVB, or pretreated with the broad-spectrum caspase inhibitor zVAD-fmk (40 μM) 20 minutes before UVB irradiation. Nuclear fragmentation was revealed after 8 hours by Hoechst staining and fluorescence microscopy. Results are shown from 1 of 5 representative experiments. (B) zVAD inhibition of UVB-induced DNA fragmentation in IDCs (as detected by propidium iodide staining and FACS analysis) at the indicated time points. Means ± SD from 3 experiments are shown. (C) IDCs were UVB irradiated (1 J/cm2), and ΔΨm was evaluated at the indicated time points by FACS analysis. UVB-induced ΔΨm loss is not inhibited by zVAD pretreatment (40 μM, 20 minutes). Means ± SD from 3 experiments are shown.
