Decreased expression of oxidative stress genes in

p45nfe2^−/−mice.(A) RT-PCR analysis of sorted Ter-119⁺ cells. Ter-119⁺ cells were isolated from the spleen and bone marrow of mice. Each lane represents pooled samples from 3 to 5 animals. RT-PCR results for the indicated genes are shown. (B) Quantitative analysis of catalase mRNA expression in Ter-119⁺ cells. Radioactive signals from the RT-PCR were quantitated by phosphoimaging analysis using MacBAS software, version 2.5. Values were normalized on the basis of results for the loading control erythropoietin receptor. Expression levels are shown relative to the signal for sample 1, which was assigned an arbitrary expression level of 1. (C) Western blot analysis of wild-type andp45nf-e2^−/− RBC extracts using a catalase-specific antibody. (D) Catalase activity in RBCs of wild-type and p45nf-e2^−/− mice.