Messenger RNA (mRNA) induction of the Gr B and perforin genes in the spleen tissue by poly I:C injection.

Mice of +/+, mi/mi, and tg/tg genotypes received an intraperitoneal injection of poly I:C. Before (0 days) and 24 hours after the injection (1 day), spleens were excised and used for RNA extraction. Five micrograms of total RNA were blotted and hybridized with the Gr B or perforin probe. Reprobing with β-actin probe verified an RNA equal loading. Similar results were yielded in 3 independent experiments.