Fig. 3.
Fig. 3. Subcellular localization of various MITFs examined by immunocytochemistry or immunoblotting. / (A) NIH3T3 cells were transfected with expression vector containing Myc-tagged +-MITF, ce-MITF, mi-MITF, orce-MITF with the NLS of SV40 large-T antigen. After 48 hours of transfection, cells were stained with anti-Myc antibody. Magnification × 4000. (B) Nuclear and cytoplasmic extracts of NIH3T3 cells transfected with the above-mentioned expression plasmid were immunoblotted with anti-Myc antibody. Extracts of cells transfected with expression vector alone were used as a negative control (shown as vec).

Subcellular localization of various MITFs examined by immunocytochemistry or immunoblotting.

(A) NIH3T3 cells were transfected with expression vector containing Myc-tagged +-MITF, ce-MITF, mi-MITF, orce-MITF with the NLS of SV40 large-T antigen. After 48 hours of transfection, cells were stained with anti-Myc antibody. Magnification × 4000. (B) Nuclear and cytoplasmic extracts of NIH3T3 cells transfected with the above-mentioned expression plasmid were immunoblotted with anti-Myc antibody. Extracts of cells transfected with expression vector alone were used as a negative control (shown as vec).

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