The effect of coexpression of +-MITF,ce-MITF, mi-MITF, or ce-MITF with NLS of SV40 large-T antigen on the luciferase activity under the control of the Gr B promoter.

Variousreporter and effector constructs were introduced into P815 cells by electroporation. Three solid squares represent CANNTG motifs between −910 and +42, ie, CAGATG (nt −521 to −516), CACGTG (nt −530 to 525), and CATTTG (nt −521 to −516) motifs. The bars represent the mean ± SE of 3 independent experiments. In some cases, the SE was too small to be shown by the bars.P < .01 by t test when compared with the control, in which expression vector containing no insert was cotransfected.