DnIKK2 inhibits the expression of cell adhesion molecules.

(A) AdV-dnIKK2–transduced (░), AdV-GFP–transduced (▪), or control (■) HUVECs were seeded in 96-well plates. AdV-dn IKK2– and AdV-GFP–transduced cells were stimulated with LPS for 6 hours and control HUVECs were left unstimulated; cells were then analyzed for expression of E-selectin, ICAM-1, VCAM-1, and ICAM-2 using a cell ELISA as described in “Materials and methods.” (B) Western blot analysis of dnIKK2, GFP-expressing, or control HUVECs. Two days after transduction with the respective viruses, cells were stimulated with LPS for 6 hours as indicated and extracts analyzed for expression of ICAM-2, IκBα, and (dn)IKK2. IKK2 was detected with an anti-IKK2 antibody to allow comparison of the levels of transfected and endogenous IKK2.