Quantitation of

MDR1 vector versus neo vector copy number in granulocytes. The two animals used were designated nos. 95E038 (A) and 95E120 (B). The copy number of MDR1 vector and neo vector sequences in peripheral blood granulocytes at different time points after transplantation is shown. Values were normalized for amplifiable DNA content by concurrent PCR for β-actin sequences, and copy number was estimated using a standard curve generated from concurrent amplification of known vector copy number DNA diluted into normal rhesus DNA.