Fig. 4.
IAP is a major adhesion receptor for immobilized TSP on SS RBCs under static conditions.
The 1F7 mAb blocked SS RBC adhesion to immobilized TSP in a dose-dependent manner under static conditions (diamonds), whereas an isotype-matched control IgG (circles) had no effect. Nearly maximal inhibition occurred at approximately 1 μg/mL 1F7 (94.5%; n = 3), and complete inhibition occurred at 4 μg/mL. The 100% adhesion represents an average of 9.6 × 105 total adherent cells/well (±SD). Neither 1F7 nor the isotype-matched control antibody had any effect on the sparse adhesion of AA RBCs to immobilized TSP observed under static conditions (data not shown).
