The circulating life spans of CD18-deficient neutrophils is not increased compared with WT.

C57BL/6-CD45.1 host animals were injected intravenously with mixtures of blood collected from WT and CD18-deficient animals. (A) The numbers of blood neutrophils (Gr-1^bright), which were of donor origin (CD45.2⁺), were determined by counting and flow cytometry at the indicated time points after injection. (B, C) The percentage of donor neutrophils (CD45.2⁺, Gr-1^bright) that were CD18-deficient (CD11a⁻) was determined by flow cytometry at the indicated time points. The data point at time 0 refers to the percentage of CD18-deficient neutrophils in the original mixture before injection into the host animal. * indicates significant (P < .05) decrease in the percentage of CD18-deficient neutrophils compared with the earlier time points. Each point represents the mean ± SEM for 4 host mice.