Fig. 6.
Fig. 6. Tyrosine phosphorylation of Jak-2 in M1/βccells with various α subunits. / (A) Immunoprecipitation–immunoblotting analysis. Cells were treated with rhGM-CSF 100 ng/mL for 10 minutes. Lysates were then subjected to immunoprecipitation with anti–Jak-2, followed by blotting with antiphosphotyrosine (top panel). The blot was then stripped and reprobed with anti–Jak-2 (bottom panel). (B) Quantitation by densitometry of tyrosine-phosphorylated Jak-2 band after rhGM-CSF treatment.

Tyrosine phosphorylation of Jak-2 in M1/βccells with various α subunits.

(A) Immunoprecipitation–immunoblotting analysis. Cells were treated with rhGM-CSF 100 ng/mL for 10 minutes. Lysates were then subjected to immunoprecipitation with anti–Jak-2, followed by blotting with antiphosphotyrosine (top panel). The blot was then stripped and reprobed with anti–Jak-2 (bottom panel). (B) Quantitation by densitometry of tyrosine-phosphorylated Jak-2 band after rhGM-CSF treatment.

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