Fig. 7.
Time course of Jak-2 tyrosine phosphorylation in M1/βc/α-1 and M1/βc/α-2 cells, after rhGM-CSF treatment.
Cells were treated with rhGM-CSF 100 ng/mL for the indicated periods, then immunoprecipitated with anti–Jak-2, followed by blotting with antiphosphotyrosine (top panel). The blot was then stripped and reprobed with anti–Jak-2 (bottom panel).