Representative Western blot, showing the effects of BCR-ABL TK inhibition and proteasome inhibition on DNA-PKcs expression in UT-7/9 BCR-ABL expressing cells and parental UT-7 cells.

Cells were grown in rhGM-CSF and exposed to tyrphostin AG957 1 μM for 3 hours (lane 4), proteasome inhibitors lactacystin (10 μM; lanes 2 and 6) and LLnl (50 μM; 8 hours' incubation; lane 3) diluted in DMSO, similar volume of DMSO was added for 8 hours as a control (lanes 1 and 5). The DNA-PKcs protein levels could be increased using both TK and proteasome inhibitors.