Fig. 6.
T-helper cell response to KLH.
B6 mice were injected with 100 μg TNP-KLH in IFA subcutaneously (left panel) or in PBS intraperitoneally (right panel) on day 0, and subsequently treated with 100 μg control immunoglobulin (○) or B7-H1Ig (▪) on days 1 and 4. The draining lymph nodes were prepared at day 7 (left panel), and the splenocytes were prepared on day 14 (right panel). The suspension cells were stimulated with KLH at the indicated concentrations. The proliferative responses of T cells to KLH were determined by the addition of 1 μCi/well3H-thymidine during the last 15 hours of the 3-day culture.
