Fig. 7.
The effect of B7-H1 on humoral response.
B6 mice at 8 per group were injected intraperitoneally with 100 μg TNP-KLH in PBS on day 0 and subsequently treated with 100 μg of control immunoglobulin (■) or B7-H1Ig (▪) on days 1 and 4. ▨, mB7-1Ig. Serum from individual mice was collected on days 7 and 14. The concentrations of anti-TNP–specific IgG of all subclasses and IgM were determined by specific sandwich ELISA as described in “Materials and methods.” The results of the OD450 reading are expressed as the mean ± SD.
