Fig. 8.
Fig. 8. Up-regulation of CD40L on B7-H1–costimulated CD4+ T cells. / Affinity-purified CD4+ T cells from B6 mice were stimulated with either immobilized control immunoglobulin, B7-H1Ig, or soluble anti-CD28 mAb in the presence of anti-CD3 mAb at either 0.2 μg/mL (A) or 1 μg/mL (B) for 4 or 24 hours. CD40L on the T cells was stained with FITC-conjugated anti-CD40L mAb and analyzed by FACS.

Up-regulation of CD40L on B7-H1–costimulated CD4+ T cells.

Affinity-purified CD4+ T cells from B6 mice were stimulated with either immobilized control immunoglobulin, B7-H1Ig, or soluble anti-CD28 mAb in the presence of anti-CD3 mAb at either 0.2 μg/mL (A) or 1 μg/mL (B) for 4 or 24 hours. CD40L on the T cells was stained with FITC-conjugated anti-CD40L mAb and analyzed by FACS.

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