Analysis of retroviral-mediated expression of γc.

(A) Schematic representation of the relative location of primers used for RT-PCR analysis of mγc expression. Primers P1 and P2 are specific for the transcripts derived from the targeted il-2rg allele in Δγc⁺-XSCID mice (knockout allele, KO). Primers P3 and P4 amplify equal fragments from the endogenous wild-type (WT allele) and retroviral-expressed mγc (MNDmγc). Primers P3 and P5 were used to specifically amplify the MNDmγc-derived transcripts. ex, exon; neo, neomycin-resistant gene cassette. (B) RT-PCR analysis of mγc transcripts. Expression levels of γc transcripts originated by RT-PCR amplification of BM samples of wild-type γc⁺, gene-corrected (mγc-BMT), and mock-treated (mock-BMT) mice are shown. Amplicons were not detected in control samples without template (NTC, lane 1) or in samples amplified in the absence of the reverse transcriptase step (RT−). Similar amplification of cyclophilin sequences demonstrated equal loading of each sample.