Immunophenotyping of thymocytes.

Thymocytes obtained from wild-type γc⁺, untreated Δγc⁺-XSCID (XSCID), and gene-corrected Δγc⁺-XSCID animals (mγc-BMT) were analyzed. Left panels show mγc expression on unfractionated thymocytes. Shown are the staining profiles of isotypic control mAbs (gray histograms) and mγc-specific mAbs (open histograms). Right panels show CD4 versus CD8 staining of thymocytes. Percentages of cells in each quadrant are shown. Results are representative of 2 independent experiments.