Comparison of cellular tyrosine phosphorylation patterns in fusion protein-transfected Ba/F3 cells and untransfected Ba/F3 cells.

(A) Comparison of cellular tyrosine phosphorylation patterns in Ba/F3.p210 cells, TEL/ARG Ba/F3 cells, and TEL/ABL Ba/F3 cells. Anti–β-actin was used as a loading control. The first and second lanes shown (Ba/F3.p210 and TEL/ARG Ba/F3, respectively) are derived from the same Western blot; the third lane shown (TEL/ABL Ba/F3) is derived from an independent Western blot performed in parallel with the first blot. (B) Comparison of cellular tyrosine phosphorylation patterns in Ba/F3.p210 cells and Ba/F3 cells. An anti-ABL antibody was used to measure levels of ABL in both cell types and to show the expression of BCR/ABL in Ba/F3.p210 cells. WB indicates Western blot.