Presence of p210^BCR/ABL in normal CD34⁺ cells inhibits adhesion of CFCs to fibronectin.

p210-eGFP– (▪) or eGFP-transduced (■) GFP⁺CD34⁺ cells (10⁴) from 4 umbilical cord blood samples were plated in 24-well plates coated with 100 μg/mL fibronectin (FN, left) or 5% BSA (right) for 2 hours. Nonadherent cells and adherent cells were collected separately and plated in methylcellulose progenitor assay. The percentage of adherent CFCs was calculated as [no. adherent CFCs/(no. adherent CFCs + no. nonadherent CFCs)] × 100. The number of CFCs in the nonadherent + adherent population was 66 ± 6/1000 cells for eGFP-transduced GFP⁺ CD34⁺ cells and 268 ± 25/1000 cells for p210-eGFP–transduced GFP⁺CD34⁺ cells. Comparison between p210-eGFP– and eGFP-transduced GFP⁺ CD34⁺ cells (P = .001).