Presence of p210^BCR/ABL in normal CD34⁺ cells enhances migration.

eGFP⁺ CD34⁺ cells were plated in fibronectin- or BSA-coated dishes and tilted to 80° for 1 hour: 10⁴normal bone marrow (n = 4) or CML bone marrow (n = 4) (A) or p210-eGFP–transduced (n = 4) or eGFP-transduced (n = 4) (B). For normal and CML bone marrow, studies were performed in the presence or absence of the blocking anti–β1-integrin antibody, P4C10. Dishes were then lowered to 20° for 12 hours, and cells that had migrated past the middle line were enumerated under the microscope. (A) Comparison between NL and CML CD34⁺ cells (P = .001). Comparison between NL CD34⁺ cells with and without P4C10 (P < .01). Comparison between CML CD34⁺ cells with and without P4C10 (P = .001). (B) Comparison between p210-eGFP– and eGFP-transduced GFP⁺CD34⁺ cells (P = .001).