Transduction of p210^BCR/ABL in NL CD34⁺ cells causes elevated levels of p27^Kip.

Cord blood CD34⁺ cells (5 × 10⁶) were transduced on 3 consecutive days with the control vector, eGFP (A: R2, eGFP⁻; R3, eGFP⁺) or with p210-eGFP (B: R3, eGFP⁻; R4, eGFP⁺). Twenty-four hours after the last transduction, cells were labeled with anti-CD34 APC, fixed, permeabilized, and incubated with antibodies against p27^Kip, and washed and incubated with phycoerythrin-conjugated goat antimouse immunoglobulin. CD34⁺ eGFP⁻ cells (C: R2, eGFP transduced; R4, p210-eGFP transduced) and CD34⁺ eGFP⁺ cells (D: R3, eGFP transduced; R5, p210-eGFP transduced) were gated, and p27^Kip levels were compared. A representative example of 3 separate experiments is shown.