Fig. 5.
IL-6 is a growth factor for plasmablasts.
(A) Activated B cells (day 0) were cultured alone or with 50 U/mL IL-2 with or without 10 ng/mL IL-6. Proliferation was determined by3H-TdR incorporation from days 0-3, 4-7, or 7-9. Experiments were performed in triplicate wells, and results are expressed as cpm ± SD. Results are representative of one out of 2 experiments. (B) At day 4, cells were incubated for 2 hours at 37°C with or without 1 mM BrdU (control). CD38-PE and BrdU-FITC staining was performed as described in “Materials and methods.” Results are representative of 1 experiment out of 2.
