American Society of Hematology

Fig. 3.

$Fig. 3. Dose response of A23187 regarding 5-LO product formation and 5-LO translocation in PMNLs. / For determination of 5-LO product formation in intact cells (A), human PMNLs (5 × 106 in 1 mL PGC buffer) were preincubated in the absence (●) or presence of 100 nM PMA (○) for 5 minutes at 37°C before stimulation with indicated concentrations ofA23187 for an additional 10 minutes. 5-LO product formation was determined by HPLC. Results are given as mean + SE; n = 3. For determination of 5-LO distribution (B), PMNLs (3 × 107in 1 mL PGC buffer) were activated with the indicated concentrations ofA23187 for 5 minutes. Cell fractionation and immunoblotting were performed as described.$

Dose response of A23187 regarding 5-LO product formation and 5-LO translocation in PMNLs.

For determination of 5-LO product formation in intact cells (A), human PMNLs (5 × 10⁶ in 1 mL PGC buffer) were preincubated in the absence (●) or presence of 100 nM PMA (○) for 5 minutes at 37°C before stimulation with indicated concentrations ofA23187 for an additional 10 minutes. 5-LO product formation was determined by HPLC. Results are given as mean + SE; n = 3. For determination of 5-LO distribution (B), PMNLs (3 × 10⁷in 1 mL PGC buffer) were activated with the indicated concentrations ofA23187 for 5 minutes. Cell fractionation and immunoblotting were performed as described.

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