Fig. 8.
![Fig. 8. [3H]arachidonic acid release in MM6 cells and human PMNLs. / MM6 cells or freshly isolated human PMNLs (2 × 106/mL in RPMI 1640 medium) were prelabeled with 0.25 μCi/mL [3H]AA for 90 minutes at 37°C and 5% CO2. After the removal of unincorporated [3H]AA, cells (5 × 106 in 1 mL PGC buffer, containing 2 mg/mL fatty acid-free albumin) were primed in the absence or presence of PMA or mezerein for 10 minutes at 37°C, A23187 was added at the indicated concentrations, and the incubations continued for another 10 minutes. Free (nonesterified) [3H]AA was determined by HPLC as described in “Materials and methods.” Results are given as mean ± SE; n = 3.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/8/10.1182_blood.v97.8.2487/5/h80810918008.jpeg?Expires=1735541546&Signature=A7NX1-ioaSnxWGrhK~SPJRBU5ybnLUimBpvl2WVg9gxWysmPH-m04Rj2ipgzkh5N0iQfWuJTV~9iwQ77ha-eFIlZlAmXDGXoR3lzPlakWnQrIxS1SJbUPN7m3znV~t9lL4OwZcKQmVt581efGqoOT~qCaNCwTUlAFPch-Kjjs6uZpBHzbJp~Y9fcQj39f5IdvqybgdizCPB1VaP5KJVu7dzV5dgspOkfaAhY2aNS1dfi6168hsatf9XuY7NGRi-ntY243tN1ysQREJKCixjLRZEolBUgB26KVP5Wl0ka1lHRnsEPiknAreASoJtiY3fQgRxg67FympD57wfl4l7dUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[3H]arachidonic acid release in MM6 cells and human PMNLs.
MM6 cells or freshly isolated human PMNLs (2 × 106/mL in RPMI 1640 medium) were prelabeled with 0.25 μCi/mL [3H]AA for 90 minutes at 37°C and 5% CO2. After the removal of unincorporated [3H]AA, cells (5 × 106 in 1 mL PGC buffer, containing 2 mg/mL fatty acid-free albumin) were primed in the absence or presence of PMA or mezerein for 10 minutes at 37°C, A23187 was added at the indicated concentrations, and the incubations continued for another 10 minutes. Free (nonesterified) [3H]AA was determined by HPLC as described in “Materials and methods.” Results are given as mean ± SE; n = 3.
