Structure and expression of the

HOXA10 retrovirus. (A) Diagrammatic representation of the integrated MSCV-HOXA10-IRES-GFP (A10-GFP) construct and the MIG (GFP) control vector. The expected sizes of the full-length viral transcripts are shown. (B) Southern blot analysis and (C) Northern blot analysis of total RNA of transduced cell lines and primary cord blood cells after 4 weeks in vitro culture. Membranes were hybridized with probes specific for GFP (Southern blot) or HOXA10 (Northern blot).