Fig. 1.
Distinction of α2 alleles A1, A2, and A3 by RFLP.
A 1332-nucleotide-long segment of intron G4 was amplified by PCR reaction (PCR product) as described in “Patients, materials, and methods.” Complete digestion of this DNA product with a combination of BglII and AseI permits the unambiguous identification of donor genotype with respect to alleles A1, A2, and A3. The expected mobilities of molecular weight standards (STDS) are indicated to the left of the gel. The size of expected digestion products are indicated to the right of the gel. Reaction products were separated on a 2% agarose gel and visualized with ethidium bromide. Digestion patterns for each possible genotype are represented.
