Detection of GUSB activity in TG donor cells against the C3H background.
(A) Cell dilutions were prepared by mixing TG and C3H spleen cells to determine the proportion of TG donor cells needed to produce a significant difference in enzyme concentrations when compared with undiluted C3H spleen cells. Error bars (SEM) are drawn on all data points but were too small to be visible on some. *Statistically significant difference (P < .05). (B) Enzyme activity is expressed as the percentage of age-matched TG tissues (100%) above untreated age-matched baseline C3H tissues (0%). Enriched (AA4.1) (▪) and nonenriched (░) uncultured TG fetal liver cells were transplanted into C3H fetuses at 1 × 106nonenriched cells and 1 × 105 enriched cells per fetus. A 50-fold enrichment was achieved, thus resulting in a 5-fold increase in the total number of hematopoietic stem and progenitor cells transplanted. *Significant (P < .05) between the enriched and nonenriched group. §Significant (P < .05) between the nonenriched and the untreated control group.
