Presence of TG donor cells in adult C3H recipient spleens after in utero transplantation.
Top row: Spleens stained for the presence of biologically active GUSB (red reaction product) after the C3H GUSB was heat-inactivated. TG indicates transgenic mouse (positive control; 20 ×); B, untreated C3H mouse (negative control; 20 ×); and TG FLCs into C3H, spleens of recipients of nonenriched TG FLCs 2 months after transplantation (20 ×). The arrows point to the areas of cells expressing enzymatically active human GUSB. Bottom row: Immunofluorescent-labeled antibodies against H-2Dd to detect TG donor cells in the recipient spleens. TG indicates transgenic mouse (positive control; 20 ×); C3H, untreated C3H mouse (negative control; 20 ×); and TG FLCs into C3H, transplant recipients with clusters of positive cells (20 ×) and with single, scattered positive donor cells (40 ×).
