Fig. 2.
Identification of the complete primary structure of Regakine-1 by amino acid sequence and by mass spectrometry analysis.
Natural Regakine-1 purified to homogeneity from serum (Figure 1) was subjected to NH2-terminal amino acid sequence analysis. Internal sequences were obtained by Edman degradation after proteolytic digestion and RP-HPLC purification. The COOH-terminal part of the sequence was evidenced by experimental COOH-terminal sequencing and mass spectrometry. X indicates unidentified residues. The numbers along the top of the figure indicate amino acid renumbering. The Regakine-1 Swiss-Prot protein database18 accession number isP82943.
