Ca⁺⁺-mobilizing agents induce expression of KSHV lytic proteins in BCBL-1 cells.

Cells were incubated with 1 μg/mL ionomycin (A-C) or 5 nM thapsigargin (D-F) for several days. At various time points, cells were harvested, labeled with Dead Red, washed, fixed, permeabilized, and labeled with anti–PF-8 or anti-gpK8.1 mAbs followed by FITC-conjugated secondary Abs. Cells were examined for fluorescence by flow cytometry. The viable cell population was examined for PF-8 (B, E) or gpK8.1 (C, F) expression, as described previously.22 Unstimulated cells (○), 1 mM EGTA (●), ionomycin or thapsigargin (▪), ionomycin or thapsigargin + EGTA (■). Data represent mean ± SEM of at least 3 separate experiments.