NK1.1 expression on thymocytes and splenocytes from control (ZAP-70^+/−) and ZAP-70^−/− mice.

Thymocytes and splenocytes were stained with PE–anti-NK1.1 and FITC–anti-CD3, or FITC–anti-TCRαβ, or with PE–anti-NK1.1 and biotin–anti-TCRγδ/streptavidin-FITC. Expressions of NK1.1 (vertical) and CD3, TCRαβ, or TCRγδ (horizontal) are illustrated. Proportions of NK1.1⁺ CD3⁻ and NK1.1⁺ CD3⁺ (left column), NK1.1⁺TCRαβ⁻ and NK1.1⁺ TCRαβ⁺(middle column), and NK1.1⁺ TCRγδ⁻, NK1.1⁺ TCRγδ⁺, and NK1.1⁻TCRγδ⁺ (right column) cells are indicated in the figures. Results are representative of 6 independent experiments.