Gene rearrangement on TCRβ loci in NK1.1⁺TCRαβ⁻ thymocytes.

A PCR was performed with genomic DNA from B6 thymocytes (Thy; left lane), B6 ear skin (middle lane), or the NK1.1⁺CD3⁻ thymocytes of ZAP-70^−/− mice (right lane) with a primer pair of a coding region of Dβ2 (Dβ2-5′) and 3′-downstream region of Jβ2.7 (Jβ2-3′) to detect rearrangements of Dβ2 to Jβ2 cluster as described in “Materials and methods.” Germline bands (G) and a rearranged band (R) are indicated.