Phenotype of spleen cells from primary FGFR3 transplant recipients.

Single-cell suspensions of spleen were prepared from primary BM transplant recipients 6 weeks after transplantation. Cell populations analyzed by flow cytometry are shown for normal littermate controls not receiving transplants (normal, ■), mice receiving empty vector-transplants (MINV, ░), and mice transplanted with MFINV-WT– (WT, ▧) or MFINV-TD– (TD, ▪) expressing BM. MFINV-TD mice displayed a decrease in both normal B (B220⁺/IgM⁺) and T (CD3⁺) lymphocytes, whereas expansions of immature B (B220⁺/IgM⁻) lymphocytes and of CD11b⁺ cells were evident. Mice receiving MFINV-WT BM displayed the same splenic cell phenotype at 6 weeks after transplantation as controls.