Fig. 2.
Localization of human hematopoiesis in NOD/SCID mice.
(A) Detection of human engraftment by flow cytometry of human CD45 in the BM and spleen of NOD/SCID mice (n = 30) transplanted with 0.6 × 106 CD34+ PBSCs and killed 2 (
), 4 (
), and 8 (▪) weeks after transplantation. (B) Detection of human engraftment in the BM and spleen of NOD/SCID mice (n = 10) by Southern blot analysis at week 8 after transplantation. Each lane (1-10) represents a single mouse. Human:mouse DNA controls are given as percent human DNA.
