Fig. 1.
Definitive hematopoiesis is impaired in Runx1- and CBFβ-deficient ES cells.
(A) Schematic diagram of the ES cell differentiation protocol. (B) Hematopoietic progenitor development in EBs from wild-type, Runx1−/−, andCbfb−/−ES cells. Colony assays were performed on EBs generated following 10 days of primary culture. The mean values (± SD) of 3 independent plates are shown from one experiment, representative of 3 independent experiments. EryP, primitive erythroid colonies; EryD, definitive erythroid colonies; mixed, erythroid/myeloid colonies. (C) Control cultures demonstrating that day 6 EBs from the experiment depicted in panel B contained primitive erythroid progenitors. Shown are total colony numbers per 106cells plated.
