Fig. 9.
Inhibition of platelet agglutination induced by echicetin-IgMκ.
Washed human platelets (500 μL, 5 × 108/mL) were stirred at 1100 rpm at 37°C. A total of 5 μg echicetin was added to the platelet suspension and incubated for 1 minute. Agglutination was started by adding 1 μg IgMκ. Monoclonal antibody against GPIb was added to platelets 2 minutes before echicetin. Curve 1: platelet agglutination induced by echicetin-IgMκ without any inhibitors. Curve 2: in the presence of 16 μg/mL SZ2. Curve 3: in the presence of 3.8 μg/mL VM16d. Curve 4: in the presence of 11.4 μg/mL VM16d.
