Marker persistence or loss in culture.

CD11b⁻ and CD11b⁺ DCs were stained for CD11b and CD14 expression immediately after sorting and after 2 days of CD40 activation. Whereas CD11b and CD14 expression is negative before and after CD40 activation of CD11b⁻ DCs, all CD11b⁺ DCs lose CD14 expression and slightly down-regulate but do not lose CD11b expression following CD40 activation. This figure is representative of 3 experiments.