American Society of Hematology

Fig. 9.

$Fig. 9. Effect of GM-CSF on thymic plasmacytoid cells. / Upon culture with GM-CSF, thymic plasmacytoid cells acquire a phenotype resembling that of thymic CD11b− DCs. Thymic plasmacytoid cells were sorted as shown in Figure 8A and cultured for 6 days, with (panel B) or without (panel A) GM-CSF. (A) After 6 days of culture with IL-3 and soluble CD40L, plasmacytoid cells differentiated into DCs and up-regulated HLA-DR, CD86, and CD83. They retained CD45RA expression and were still negative for CD45RO and CD11c. (B) When cultured with IL-3, soluble CD40L, and GM-CSF, plasmacytoid-derived DCs expressed higher levels of CD86 and CD83. A fraction down-regulated CD45RA and acquired CD11c expression. This figure is representative of 3 experiments.$

Effect of GM-CSF on thymic plasmacytoid cells.

Upon culture with GM-CSF, thymic plasmacytoid cells acquire a phenotype resembling that of thymic CD11b⁻ DCs. Thymic plasmacytoid cells were sorted as shown in Figure 8A and cultured for 6 days, with (panel B) or without (panel A) GM-CSF. (A) After 6 days of culture with IL-3 and soluble CD40L, plasmacytoid cells differentiated into DCs and up-regulated HLA-DR, CD86, and CD83. They retained CD45RA expression and were still negative for CD45RO and CD11c. (B) When cultured with IL-3, soluble CD40L, and GM-CSF, plasmacytoid-derived DCs expressed higher levels of CD86 and CD83. A fraction down-regulated CD45RA and acquired CD11c expression. This figure is representative of 3 experiments.

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